Related Experiment Video
Updated: Jun 6, 2026
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Published on: August 29, 2018
[Dynamic changes of Smac/DIABLO protein expression in rat donor myocardium suffered from hypothermic preservation]
Ming-zhi Zheng1, Jian-ping Jiang, Lin Fu
1Zhejiang Medical College, Hangzhou 310053, China.
Insights
Hypothermic preservation affects heart function. Prolonged preservation increased Smac/DIABLO protein expression and cardiomyocyte apoptosis, potentially leading to heart malfunction.
Area of Science:
- Cardiology
- Cell Biology
- Biochemistry
Background:
- Hypothermic preservation is crucial for cardiac transplantation.
- Understanding molecular changes during preservation is vital for improving outcomes.
Purpose of the Study:
- To investigate the impact of varying hypothermic preservation durations on Smac/DIABLO protein expression in rat hearts.
- To correlate preservation time with oxidative stress and apoptosis markers.
Main Methods:
- Isolated rat hearts were preserved using the Langendorff model in Celsior solution at 4°C for 0-12 hours.
- Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured.
- Cell apoptosis was assessed using TUNEL assay, and Smac/DIABLO protein expression was analyzed via Western blotting.
Main Results:
- Hypothermic preservation led to a time-dependent decrease in SOD activity and increase in MDA content.
- Apoptotic cell percentage increased with prolonged preservation.
- Smac/DIABLO protein expression initially rose at 3-6 hours but declined after 9 hours of preservation.
Conclusions:
- Extended hypothermic preservation may upregulate Smac/DIABLO protein expression.
- This upregulation could contribute to cardiomyocyte apoptosis and subsequent cardiac dysfunction.
Aim:
To investigate the effect of different duration of hypothermic preservation on the expression of Smac/DIABLO protein in rat hearts.
Methods:
The Langendorff model of isolated rat heart was used. After stored in 4 degrees C Celsior solution for different time (0, 3, 6, 9 or 12 h), the activity of SOD and the content of MDA in heart mitochondria were measured. Cell apoptosis was detected by TUNEL technique. The expression of Smac/DIABLO protein was also analyzed by Western blotting.
Results:
(1) After hypothermic preservation, the activity of SOD was decreased and the content of MDA was increased in rat hearts in a time-dependent manner. (2) Prolonged the hypothermic preservation, the percentage of apoptotic cell also enhanced. (3) After long-term of cold preservation, the expression of Smac/DIABLO protein increased at 3-6 h of preservation but decreased after 9 h.
Conclusion:
Prolonged the hypothermic preservation might lead to the expression of Smac/DIABLO protein and induce cardiomyocytes apoptosis, which may in turn result in malfunction of cardiomyocytes.
