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Updated: Jun 6, 2026
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
Published on: December 28, 2015
[Establishment of a HEK293 cell line stably expressing human HCN2 gene]
Xin Zhao1, Xiang-Jun Yang, Xia Bai
1Department Cardiovascular Research, The First Affiliated Hospital to Suzhou University, Suzhou 215006, China.
Aim:
To create a model for studying ionic channels by means of the expressing human HCN2 and G418-resistant HEK293 cell lines established.
Methods:
pcDNA3-hHCN2 was transfected with Lipofectin2000 into HEK293 cell line. The transfected cells would be survived in the further culture medium containing G418 antibiotic as the hHCN2 gene could express a G418 resistant products. Whole-cell patch clamp investigated that hHCN2 gene was transfected into HEK293 cells.
Results:
The G418 resistant (600 ug/ml) HEK293 cell line was established successfully and whole-cell patch clamp recorded ionic currents of transfected hHCN2.
Conclusion:
The G418 resistant HEK293 cell line was successfully established with transfection of plasmid pcDNA3-hHCN2 by Lipofectin, which might be useful for studying the relationship between the structure and function of cloned ionic channels.
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