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Super-resolution Fluorescence Microscopy

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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Nanoparticles for highly efficient multiphoton fluorescence bioimaging.

Laura Martinez Maestro¹, Emma Martín Rodriguez, Fiorenzo Vetrone

¹GIEL, Departamento de Física de Materiales, Universidad Autónoma de Madrid, 8049 Madrid, Spain.

|December 18, 2010

Summary

This summary is machine-generated.

New fluoride-based inorganic upconverting nanoparticles (NaYF4:Er3+, Yb3+) show superior multiphoton fluorescence efficiency. These advanced nanoparticles outperform current nanoprobes for bioimaging applications.

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Area of Science:

Materials Science
Nanotechnology
Biomedical Imaging

Background:

Multiphoton fluorescence imaging offers advantages for deep tissue visualization.
Current inorganic fluorescent nanoprobes like CdSe quantum dots and gold nanorods have limitations in efficiency.
Development of novel nanomaterials is crucial for advancing bioimaging techniques.

Purpose of the Study:

To introduce and characterize a new class of fluoride-based inorganic upconverting nanoparticles.
To evaluate the multiphoton excited fluorescence efficiency of NaYF4:Er3+, Yb3+ nanoparticles.
To compare their performance against existing nanoprobes for bioimaging.

Main Methods:

Synthesis of NaYF4:Er3+, Yb3+ fluoride-based inorganic upconverting nanoparticles.
Measurement of near-infrared-to-visible photon conversion efficiency.
Comparative analysis with CdSe quantum dots and gold nanorods using multiphoton fluorescence microscopy.

Main Results:

NaYF4:Er3+, Yb3+ nanoparticles demonstrate unprecedented multiphoton excited fluorescence efficiency.
Their conversion efficiency significantly exceeds that of CdSe quantum dots and gold nanorods.
These nanoparticles represent a breakthrough in fluorescent nanoprobe technology.

Conclusions:

Fluoride-based inorganic upconverting nanoparticles (NaYF4:Er3+, Yb3+) are the most efficient multiphoton excited fluorescent nanoparticles developed.
These findings pave the way for enhanced multiphoton fluorescence bioimaging and fluorescence tomography.
This research offers a promising alternative to current nanoprobes for advanced biomedical applications.