Lipid peroxidation in the liver and lungs in SiO(2)-induced granulomatosis

LPO activity in the liver and lungs of Wistar rats during the development of granulomatous inflammation caused by intravenous injection of SiO(2)microparticles was evaluated by the content of conjugated dienes, ketodienes, conjugated trienes, and products of interaction of LPO intermediates with TBA. In the lungs, changes in LPO activity manifested in increased content of ketodienes and conjugated trienes (by 1.6 and 1.5 times on days 3 and 14 after injection of SiO(2)microparticles, respectively) and conjugated dienes (by 1.2 times on day 21) compared to the control levels, but the content of TBA-reactive substances remained within the normal range. In the liver, the content of conjugated dienes increased by 1.6 times and that of ketodienes and conjugated trienes by 2.3 times; the content of TBA-reactive substances increased by 1.7 times compared to the control. The content of TBA-reactive substances in the liver gradually increased and by day 14 this parameter surpassed the control level by 3.9 times, but on day 21 it returned to normal. Thus, LPO processes in the liver after injection of SiO(2)microparticles were more intensive than in the lungs and their activity underwent phasic changes.