Effect of cAMP on peptide formation in human erythrocytes depending of cell age

We found that exogenous and endogenous cAMP induces changes in the spectrum and amount of intraerythrocytic peptides in human erythrocytes of different age. Activation of cAMP formation with epinephrine leads to the appearance of peptides of the other type and to an increase in their total amount in young cells, while blockade of these β-adrenoreceptors with propranolol eliminates these effects. Inhibition of cAMP phosphodiesterase with imidazole in the absence of hormonal signals elevates the content of longer peptides in erythrocytes compared to the effect of exogenous cAMP. The degree of this elevation depends on erythrocyte age.