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Updated: Jun 5, 2026
Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
Published on: May 4, 2015
[Hypoxic preconditioning increase nPKCepsilon membrane translocation in the brain of mice]
Xue-mei Wang1, Jun-fa Li, Peng-yu Zu
1Department of Neurobiology, Capital University of Medical Sciences, Beijing Key Laboratary for Neural Regeration and Repairing, Beijing 100054, China.
Aim:
To explore the role of novel protein kinases C (nPKCs) in the development of cerebral hypoxic preconditioning.
Methods:
By using the mice model of hypoxic preconditioning, which was established before in our lab, the biochemistry techniques of SDS-PAGE and Western blot were applied to observe the effects of repetitive hypoxic exposure (H0-H4) on nPKCs (nPKCepsilon, delta, eta, mu and theta) membrane translocation in hippocampus and cortex.
Results:
nPKCepsilon membrane translocation was increased in response to the hypoxic exposure times in the hippocampus (H0: 41.6% +/- 1.4% vs. H1-H4: 46.9% +/- 4.5%, 52.7% +/- 3.9%, 58.8% +/- 2.7% and 61.3% +/- 3.7%) and cortex (H0: 38.4% +/- 4.5% vs. 42.4% +/- 5.0%, 48.7% +/- 6.5%, 55.3% +/- 8.9% and 61.2% +/- 10.2%) of mice, and there were statistic significances among H2, H3 and H4 in hippocampus, and H3 and H4 in cortex respectively (P < 0.01). But for nPKCdelta, eta, mu and theta membrane translocation, there were no any significant changes in hippocampus and cortex of hypoxic preconditioned mice.
Conclusion:
nPKCepsilon may play an important role in the development of cerebral hypoxic preconditioning, but it need more evidence to prove.
