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Updated: Jun 5, 2026
An Experimental System to Study Mechanotransduction in Fetal Lung Cells
Published on: February 16, 2012
[Influence of vasoactive intestinal peptide on expression of pulmonary surfactant associated protein A in lung
Lian Li1, Zi-qiang Luo, Gan-qiou Wu
1Department of Physiology, Xiang Ya School of Medicine, Central South University, Changsha 410078, China.
Aim:
To study the influence of VIP on the expression of SP-A and its intracellular signal transduction pathway.
Methods:
The influence of VIP on the expression of SP-A was studied by immunohistochemistry and RT-PCR. The intracellular signal transduction pathway was further investigated by using receptor antagonist, protein kinase inhibitor and antisense oligonucleotides.
Results:
(1) VIP(10(-8) mol/L) enhanced SP-A protein expression in alveolar type II cells (ATII) and increased the content of SP-A mRNA in lung tissue. (2) VIP receptor antagonist [D-P-C1-Phe (6)-Leu (17)]-VIP (10(-6) mol/L) could suppress the VIP-induced expression of SP-A protein and SP-A mRNA. (3) c-fos antisense oligonucleotides (9 x 10(-6) mol/L) could inhibit the VIP-induced expression of SP-A protein and SP-A mRNA. (4) Protein kinase C(PKC) inhibitor H7 (10(-5) mol/L) could also depress the V1P-induced SP-A protein and SP-A mRNA.
Conclusion:
VIP can up-regulate the expression of SP-A through its receptor. PKC and c-fos protein play important roles in the intracellular signal transduction pathway through which VIP induces the expression of SP-A.
