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Updated: Jun 5, 2026
Functional Characterization of Endogenously Expressed Human RYR1 Variants
Published on: June 9, 2021
[Expression of ryanodine receptor isoforms in airway smooth muscle cells of rats]
Ying Wang1, Sui-yang Zhang, Xi-liang Wang
1Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Aim:
To detect the expression of ryanodine receptor (RyR) subtypes in normol rat airway smooth muscle cells(ASMCs) and changes during chronic asthma formation.
Methods:
ASMCs were cultured with collagen enzyme digestion method. The expression of subtypes of RyR were detected by RT-PCR. Purified PCR product linked with pGEM-T vector to make DNA sequence assay. Chronic asthma model was made with OVA, the changes of RyRs detected by RT-PCR.
Results:
All subtypes of RyR were expressed in airway smooth muscle cells of normol rat. The expression of RyR1 increased obviously compared with control group (P < 0.05) on chronic asthma.
Conclusion:
Co-expression of three subtypes of RyR in ASMCs of normal rat, indicate that there are complicated intercellular Ca2+ regulation mechanism in ASM, moreover RyR1 might play a role during asthma development.
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