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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
Highly sensitive electrochemiluminescent biosensor for adenosine based on structure-switching of aptamer
Xi Zhu1, Yashan Zhang, Weiqiang Yang
1Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Analytica Chimica Acta
|December 21, 2010
Summary
A novel electrochemiluminescent (ECL) biosensor was developed for sensitive adenosine detection. This DNA-based sensor offers high selectivity and reusability for adenosine determination.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Nanotechnology
Background:
- Adenosine detection is crucial in various biological and medical applications.
- Developing sensitive and selective biosensors is essential for accurate adenosine quantification.
- Existing methods may lack the required sensitivity or selectivity for certain applications.
Purpose of the Study:
- To develop a highly sensitive and selective electrochemiluminescent (ECL) biosensor for adenosine determination.
- To utilize DNA immobilization and aptamer recognition for signal generation.
- To evaluate the performance of the developed biosensor in terms of sensitivity, selectivity, and reusability.
Main Methods:
- Immobilization of capture DNA on a gold electrode via Au-thiol interaction.
- Modification of the electrode with Ru-SNPs-labeled DNA containing an adenosine aptamer.
- Detection of adenosine based on the displacement of Ru-SNPs and subsequent ECL signal change.
- Characterization of the biosensor's performance, including detection limit and selectivity.
Main Results:
- The ECL biosensor demonstrated high sensitivity for adenosine detection within a concentration range of 1.0×10(-10) to 5.0×10(-6)molL(-1).
- A low detection limit of 3.0×10(-11)molL(-1) was achieved.
- The biosensor exhibited excellent selectivity towards adenosine, with minimal interference from other nucleosides.
- The developed biosensor showed good reusability, retaining 91% of its original ECL signal after 30 uses.
Conclusions:
- A novel and effective ECL biosensor for adenosine determination has been successfully developed.
- The biosensor offers significant advantages in terms of sensitivity, selectivity, and reusability.
- This approach holds promise for sensitive and reliable adenosine detection in various analytical and diagnostic applications.
