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Updated: Jun 5, 2026
Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
Published on: October 30, 2018
[The transfection and expression of retro viral-mediated human FL cDNA in bone marrow stromal cell line]
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
Aim:
The expression of retroviral-mediated FL gene transfer into bone marrow stromal cell line HFCL was studied.
Methods:
FLT3 ligand (FL) cDNA was recombined with retroviral vector pLXSN by gene recombination technology. The recombinant plasmid was transferred into retrovirus packaging cell line PA3 17 by lipofectamine, and the resistant clones were selected by G418 selective medium. The mRNA expression in HFCL cells and integration of genome DNA were assayed by RT-PCR and genomic DNA PCR. The biological activity of FL in the culture was investigated by mouse bone marrow CFU-GM assay.
Results:
The recombinant plasmid pLFSN was successfully constructed. The expression of FL mRNA was detected in HFCL cells. In the genome of these infected target cells, neo gene and FL cDNA were successfully expressed. The biological activity of FL in the culture demonstrated that HFCL cells transfected with FL could significantly augment FL in vitro.
Conclusion:
These results suggest that bone marrow stromal cell lines might become target cells of gene therapy.
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