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Updated: Jun 5, 2026
A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
Published on: November 14, 2020
[A comparative study on three models of co-culture of neurons and astrocytes]
1Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To find a better method for harvesting highly purified neurons by comparing three methods used for co-culture of neurons and astrocytes.
Methods:
The co-culture models of neurons and astrocytes were established by primary culture, Banker's co-culture method or Transwell cell-culture inserts. The neurons and astrocytes cultured in vitro were from neonatal rats.
Results:
The highly purified neurons were not harvested by primary culture because the neurons and astrocytes grew on the same cover slip and it was difficult to control the growth velocity of astrocytes. The highly purified neurons were harvested by Banker's co-culture method or the method using Transwell cell-culture inserts, but the procedure of the former was more complicated than that of the later.
Conclusions:
The culture method using Transwell cell-culture inserts is recommended for the establishment of the co-culture system of neurons and astrocytes.
