Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A unimolecular GLP-1 and FGF21 dual agonist for treatment of metabolic dysfunction-associated steatohepatitis.

Communications medicine·2026

Same author

The Polymers of Life: Exploring Cellular Function Through Polymer Concepts.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Real-time processing of high-throughput quantitative phase microscopy data using a Jetson Orin Nano.

Biophotonics discovery·2026

Same author

Soaking Up Success: Sponge-Assisted Nanoparticle Transfection.

Research square·2026

Same author

Genetically Encoded Sterol-Modification of a Synthetic Intrinsically Disordered Protein Drives Its Self-Assembly Into Diverse Morphologies.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Enhanced penetration depth in optical coherence tomography and photoacoustic microscopy <i>in vivo</i> enabled by absorbing dye molecules.

Optica·2026

Search research articles

Related Experiment Video

Updated: Jun 5, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Published on: March 28, 2014

Plasmonic flow cytometry by immunolabeled nanorods.

Matthew J Crow¹, Stella M Marinakos, J Michael Cook

¹Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|December 25, 2010

Summary

Noble metal nanoparticles offer a powerful new way to detect cell surface receptors in flow cytometry. This plasmonic method significantly enhances signal detection compared to traditional fluorescence methods.

More Related Videos

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Published on: March 5, 2021

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Related Experiment Videos

Last Updated: Jun 5, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Published on: March 28, 2014

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Published on: March 5, 2021

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Area of Science:

Biophysics
Nanotechnology
Cell Biology

Background:

Fluorescence flow cytometry is a standard technique for measuring cellular characteristics like receptor expression.
Cell surface receptors are typically labeled using fluorescent antibodies or ligands.

Purpose of the Study:

To introduce and demonstrate the utility of plasmonic nanoparticles for cell surface receptor labeling in flow cytometry.
To compare the performance of gold nanorod-based labeling with traditional fluorescence-based methods.

Main Methods:

Functionalized noble metal nanoparticles, specifically gold nanorods, were conjugated to antibodies targeting cell surface receptors.
Cells labeled with nanorod-conjugated antibodies were analyzed using flow cytometry, measuring light scattering.
The scattering signal from nanorod-labeled cells was compared to the fluorescence signal from conventionally labeled cells.

Main Results:

Gold nanorod-based plasmonic flow cytometry demonstrated significantly higher scattering intensity compared to fluorescence intensity.
A six-fold increase in scattering was observed for cells labeled with anti-epidermal growth factor receptor (EGFR)-conjugated nanorods versus IgG1 controls.
Effective labeling was achieved using a 1,000-fold lower antibody concentration with nanorods compared to fluorescent labels.

Conclusions:

Plasmonic nanoparticles provide a highly sensitive and efficient alternative for cell surface receptor detection in flow cytometry.
The spectral tunability and strong scattering properties of gold nanorods offer advantages over traditional fluorescent labels.
This approach holds potential for improved cellular analysis and diagnostics.