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Related Experiment Video

Updated: Jun 5, 2026

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
07:28

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

Published on: March 23, 2020

Multicolor Brainbow imaging in zebrafish.

Y Albert Pan, Jean Livet, Joshua R Sanes

    Cold Spring Harbor Protocols
    |January 6, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This study details the Brainbow imaging technique for labeling neurons with multiple fluorescent proteins. This method allows researchers to distinguish and trace individual neurons, even in densely packed tissues like the zebrafish trigeminal sensory ganglion.

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    Published on: February 10, 2021

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    Last Updated: Jun 5, 2026

    Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
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    Published on: March 23, 2020

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    06:27

    In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy

    Published on: April 28, 2023

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    05:25

    In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

    Published on: February 10, 2021

    Area of Science:

    • Neuroscience
    • Molecular Biology
    • Genetics

    Background:

    • Distinguishing individual neurons in complex neural circuits is challenging.
    • Multi-color labeling strategies are needed to trace neuronal processes effectively.

    Purpose of the Study:

    • To describe a protocol for utilizing the Brainbow strategy for multi-color neuronal labeling.
    • To demonstrate the application of Brainbow imaging in zebrafish for tracing axonal processes.

    Main Methods:

    • Employing the Brainbow system, which relies on random Cre/lox recombination.
    • Generating diverse combinations of red, blue, and green fluorescent proteins within individual cells.
    • Applying the technique to the zebrafish trigeminal sensory ganglion.

    Main Results:

    • Successful multi-color labeling of neurons was achieved.
    • The color variations enabled clear differentiation and tracing of individual axonal processes.
    • Demonstrated the utility of Brainbow for visualizing neuronal morphology in zebrafish.

    Conclusions:

    • The Brainbow strategy provides a powerful tool for neuronal tracing in zebrafish.
    • This protocol can be adapted for broader applications in neuroscience research.
    • Color-based neuronal tracing enhances the study of neural circuit connectivity.