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Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
Published on: October 19, 2021
Immature embryo rescue and culture
Xiuli Shen1, Fred G Gmitter, Jude W Grosser
1IFAS, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2011
Summary
Immature embryo rescue effectively recovers triploid hybrids from interploid crosses, overcoming early embryo abortion. This technique is crucial for developing seedless fruits and advancing plant breeding programs.
Area of Science:
- Plant Science
- Reproductive Biology
- Horticulture
Background:
- Embryo culture techniques are vital for plant breeding and physiological studies.
- Immature embryo rescue overcomes inviability and dormancy in sexual crosses.
- Interploid hybridization aims to combine traits for improved seedless fruits, but faces crossing barriers.
Purpose of the Study:
- To discuss the application of interploid crosses in citrus cultivar improvement.
- To illustrate successful protocols for immature embryo rescue and culture.
- To identify factors influencing the success of embryo rescue for triploid progeny.
Main Methods:
- Utilizing interploid crosses between diploid and allotetraploid citrus parents.
- Implementing immature embryo excision and in vitro culture.
- Detailing protocols from embryo isolation to greenhouse recovery.
Main Results:
- Demonstrated successful recovery of triploid hybrids that fail to develop in vivo.
- Provided comprehensive protocols adaptable to other plant species.
- Identified key factors affecting triploid progeny recovery.
Conclusions:
- Immature embryo rescue is a viable strategy for overcoming hybridization barriers in polyploid breeding.
- This method facilitates the development of improved seedless fruits through interploid crosses.
- The presented protocols offer a valuable resource for plant breeding programs.
