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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 5, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Fluorescent cell barcoding for multiplex flow cytometry.

Peter O Krutzik1, Matthew R Clutter1, Angelica Trejo1

  • 1Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, California.

Current Protocols in Cytometry
|January 6, 2011
PubMed
Summary
This summary is machine-generated.

Fluorescent cell barcoding (FCB) streamlines flow cytometry by labeling cells with unique fluorescent dyes. This method enhances data quality and reduces reagent use for high-throughput biological analysis.

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Last Updated: Jun 5, 2026

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry is a powerful tool for cell analysis.
  • High-throughput and high-content analysis require efficient sample processing.
  • Current methods can be limited by reagent consumption and data variability.

Purpose of the Study:

  • To describe the application of Fluorescent Cell Barcoding (FCB) for multiplexed flow cytometry.
  • To detail the benefits of FCB in terms of efficiency and data quality.
  • To provide technical guidance for implementing FCB in various biological samples.

Main Methods:

  • Cells are labeled with unique combinations of fluorescent dyes (barcoding).
  • Barcoded samples are pooled before antibody staining and flow cytometry acquisition.
  • Technical considerations including dye selection, buffers, compensation, and software analysis are discussed.

Main Results:

  • FCB enables multiplexing of samples for simultaneous analysis.
  • Significant reduction in antibody consumption (10- to 100-fold) is achieved.
  • Improved data robustness by minimizing pipetting errors and staining variations.
  • Enhanced acquisition speed facilitates large-scale profiling experiments.

Conclusions:

  • FCB is an effective method for high-throughput, high-content flow cytometry.
  • The technique offers substantial cost savings and improved experimental consistency.
  • FCB is applicable to both cell lines and primary peripheral blood samples.