[Expression and function analysis of chemokine receptor CCR6 in HEK293 cells]
Wei Wei1, Lan Gao, Fei-fei Zhang
1School of Life Sciences, Lanzhou University, Lanzhou 730000, China. weiweiw04@163.com
Aim:
To construct a cell line which stably expresses human chemokine receptor 6 (CCR6).
Methods:
The human CCR6 cDNA and plasmid G were co-transfected into HEK 293 cells and the clones stably expressing CCR6 were picked out. The expression of CCR6 in HEK293 cells was detected by RT-PCR, Western blotting, immunofluorescence test, calcium mobilization and in vitro chemotaxis assay.
Results:
The transfected HEK293 cells could stably express functional human CCR6.
Conclusion:
Successfully establish a cell line which stably express human CCR6 and lays the foundation for its biological function's study and specific antagonist screening.
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