Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rh Blood Group01:19

Rh Blood Group

The Rhesus (Rh) antigen is crucial in determining blood groups and ensuring compatibility during blood transfusions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rh immune globulin: an interfering substance in compatibility testing.

Immunohematology·2019
Same author

DEL phenotype.

Immunohematology·2017
Same author

Bloody Brilliant: A History of Blood Groups and Blood Groupers.

Immunohematology·2017
Same author

Immunosuppressive protocols for transplantation and certain hematologic malignancies can prevent the primary immune response to the D blood group antigen.

Immunohematology·2013
Same author

Education in transfusion medicine for medical students and doctors.

Vox sanguinis·2013
Same author

Gene ontology study of methyl jasmonate-treated and non-treated hairy roots of Panax ginseng to identify genes involved in secondary metabolic pathway.

Genetika·2010

Related Experiment Video

Updated: Jun 5, 2026

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
09:04

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

Laboratory methods for Rh immunoprophylaxis: a review.

S G Sandler1, S Sathiyamoorthy

  • 1Georgetown University Hospital, Room M1306, Department of Laboratory Medicine, 3800 Reservoir Road, NW, Washington, DC 20007, USA.

Immunohematology
|January 11, 2011
PubMed
Summary

Accurately measuring fetal Rh-positive red blood cells (RBCs) in D-negative mothers is crucial for Rh immune globulin dosing. Current methods like the rosette screen and Kleihauer-Betke assay have limitations, while flow cytometry offers precision but limited accessibility.

More Related Videos

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
06:34

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Published on: December 1, 2017

Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues
08:42

Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues

Published on: October 26, 2021

Related Experiment Videos

Last Updated: Jun 5, 2026

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
09:04

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
06:34

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Published on: December 1, 2017

Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues
08:42

Immunohistochemistry Test for the Lyssavirus Antigen Detection from Formalin-Fixed Tissues

Published on: October 26, 2021

Area of Science:

  • Obstetrics and Gynecology
  • Hematology
  • Clinical Laboratory Science

Background:

  • Rh immune globulin (RhIG) prophylaxis requires accurate estimation of fetomaternal hemorrhage (FMH).
  • Estimating FMH volume relies on quantifying fetal red blood cells (RBCs) in maternal circulation.
  • Distinguishing fetal from maternal RBCs has historically used blood type or hemoglobin differences.

Purpose of the Study:

  • To review laboratory methods for detecting and quantifying fetal RBCs in maternal blood.
  • To assess current laboratory practices for FMH detection in US hospitals.
  • To evaluate the suitability of different methods for Rh immunoprophylaxis.

Main Methods:

  • Literature review of laboratory methods for fetal RBC detection.
  • Analysis of College of American Pathologists Fetal RBC Detection Survey data.
  • Evaluation of rosette screen, acid-elution (Kleihauer-Betke) assay, and flow cytometry.

Main Results:

  • The rosette screen is widely used for D-negative mothers but unsuitable for D-positive mothers or unknown fetal D type.
  • The Kleihauer-Betke assay is sensitive but tedious, inaccurate, and difficult to reproduce.
  • Flow cytometry provides precise quantification but is not widely available due to logistical and fiscal barriers.

Conclusions:

  • Current laboratory methods for FMH assessment present significant limitations for optimal Rh immune globulin dosing.
  • The limitations of existing methods highlight a need for more accessible and accurate techniques.
  • Flow cytometry shows promise for precise quantification but requires broader implementation for clinical utility.