Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Bone Matrix01:18

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
Essential Minerals for Bone Health01:31

Essential Minerals for Bone Health

The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Bone Remodeling and Repair01:31

Bone Remodeling and Repair

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering bio-selectivity: Novel denture coatings for targeted inhibition of denture-associated pathogens.

Dental materials journal·2026
Same author

Immune-instructive biomaterials in oral tissue regeneration and therapy.

Biomaterials·2026
Same author

Resilient nanostructured bioanalytic microneedle longitudinally monitors preclinical renal and hepatic drug clearance and dysfunction.

Science translational medicine·2026
Same author

Next-Generation Orthodontics: Functional Resins, Biomechanics, Biocompatibility, and Current Clinical Reality of Direct 3D-Printed Aligners.

Journal of functional biomaterials·2026
Same author

Dedifferentiated Adipocytes Improve Heart Function Post-Myocardial Infarction.

Journal of regenerative medicine·2026
Same author

Deep Generative AI for Multi-Target Therapeutic Design: Toward Self-Improving Drug Discovery Framework.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jun 5, 2026

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
05:41

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

Published on: February 23, 2017

Osteoblast interactions within a biomimetic apatite microenvironment.

Eric J Tsang1, Christopher K Arakawa, Patricia A Zuk

  • 1Department of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, 5121 Engineering V, Los Angeles, CA 90095, USA.

Annals of Biomedical Engineering
|January 15, 2011
PubMed
Summary

Accelerated apatites influence osteoblast behavior. Proteins are crucial for cell attachment to apatite, and phosphate uptake may be key to cell survival in this microenvironment.

More Related Videos

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

Related Experiment Videos

Last Updated: Jun 5, 2026

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
05:41

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

Published on: February 23, 2017

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Biomineralization

Background:

  • Accelerated apatites are known to promote osteoblastic differentiation and bone formation.
  • The precise mechanisms of cell interaction within the apatite microenvironment are not fully understood.

Purpose of the Study:

  • To investigate the in vitro interactions between osteoblasts (MC3T3-E1 cells) and the apatite microenvironment.
  • To elucidate the role of serum proteins and ion transport in these interactions.

Main Methods:

  • Evaluation of MC3T3-E1 cell attachment, spreading, and viability on apatite surfaces.
  • Experiments conducted with and without serum proteins.
  • Treatment with phosphate and calcium transport inhibitors.

Main Results:

  • Serum proteins are critical for MC3T3-E1 cell adherence and survival on apatite surfaces.
  • In the absence of protein, cell viability decreased significantly, but could be rescued by phosphate transport inhibitors.
  • Apatite surfaces rapidly depleted extracellular calcium (Ca2+) and phosphate (PO43-) ions from the culture medium.

Conclusions:

  • The biomimetic apatite surface significantly alters the osteoblast microenvironment.
  • Altered ion concentrations and protein interactions on apatite surfaces impact osteoblast survival and differentiation potential.