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Updated: Jun 5, 2026

A Microscopic 2,3,5-Triphenyltetrazolium Chloride Assay for Accurate and Reliable Analysis of Myocardial Injury
Published on: November 28, 2025
Lipid abnormalities in myocardial cell injury
1Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
Abstract:
Considerable evidence indicates that abnormalities in fatty acid and phospholipid metabolism are important in the pathogenesis of the membrane dysfunction that leads to irreversible myocardial cell injury during myocardial ischemia and related conditions. Membrane dysfunction is mediated by phospholipid degradation and by the accumulation of amphipathic lipid species, including free fatty acids, long-chain acyl-coenzyme A esters, long-chain acylcarnitines, and lipid peroxides. Accumulation of free arachidonic acid, a fatty acid normally stored in membrane phospholipids, is a sensitive indicator of phospholipid degradation. Ongoing work is aimed at defining mechanisms of the phospholipid alterations that appear to involve phospholipase-mediated phospholipid catabolism and impaired phospholipid synthesis.
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