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Related Experiment Videos

Antigen-presenting cells for CD8+ T cells.

J Sprent1, M Schaefer

  • 1Department of Immunology, IMM4A, Research Institute of Scripps Clinic, La Jolla, California 92037.

Immunological Reviews
|October 1, 1990
PubMed
Summary
This summary is machine-generated.

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Various cell types can present antigens to CD8+ T cells without help. Antigen-presenting cell (APC) function depends on factors like antigen density and cell interactions, influencing T cell responses.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T cell activation typically requires antigen presentation by specialized antigen-presenting cells (APCs) with co-stimulatory signals.
  • The capacity of diverse cell types to act as APCs and the factors influencing their function remain areas of active investigation.

Purpose of the Study:

  • To investigate the ability of various cell types to present class I alloantigens to CD8+ T cells independently of external help.
  • To identify factors that enhance or restore antigen-presenting cell (APC) function in different cell types.

Main Methods:

  • Utilized purified unprimed CD8+ T cells and various potential antigen-presenting cells (APCs), including dendritic cells, spleen cells, peritoneal exudate cells, and T-tumor lines.
  • Assessed APC function by measuring T cell responses in the presence or absence of added lymphokines.

Related Experiment Videos

  • Manipulated APCs using lipopolysaccharide, neuraminidase (N'dase), and interferon-gamma (IFN-gamma) to evaluate their impact on APC function.

    • Main Results:

    • Multiple cell types, including dendritic cells and certain T-tumor lines, presented class I alloantigens to CD8+ T cells without added help.
    • Some cell types, like T-blast cells and overnight-adherent peritoneal exudate cells (OA-PEC), required lymphokines for APC function, which could be enhanced by lipopolysaccharide or neuraminidase (N'dase) treatment.
    • Neuraminidase (N'dase) treatment partially restored APC function in resting T stimulators, but strong responses still necessitated exogenous lymphokines.
    • Interferon-gamma (IFN-gamma) treatment of T-tumor lines indicated a correlation between APC function and class I antigen expression.

    Conclusions:

    • Antigen-presenting cell (APC) function is influenced by multiple factors, including antigen density, accessory molecule levels, and surface charge.
    • The avidity of the interaction between T cells and APCs is critical, with high avidity leading to helper-independent responses and lower avidity resulting in helper-dependent responses.