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Updated: Jun 5, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Fluorescence-based detection of nitric oxide in aqueous and methanol media using a copper(II) complex
Biplab Mondal1, Pankaj Kumar, Pokhraj Ghosh
1Department of Chemistry, Indian Institute of Technology Guwahati, Assam 781039, India. biplab@iitg.ernet.in
Abstract:
The quenched fluorescent intensity of a copper(II) complex, 1, of a fluorescent ligand, in degassed methanol or aqueous (buffered at pH 7.2) solution, was found to reappear on exposure to nitric oxide. Thus, it can function as a fluorescence based nitric oxide sensor. It has been found that the present complex can be used to sense nanomolar quantities of nitric oxide in both methanol and pH 7.2 buffered-water medium.

