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UF-1000i flow cytometry is an effective screening method for urine specimens
Kamran Kadkhoda1, Kanchana Manickam, Pat Degagne
1Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, R3E 0J9 Manitoba, Canada.
Diagnostic Microbiology and Infectious Disease
|January 22, 2011
Summary
The UF-1000i™ flow cytometer efficiently screens urine samples for bacteria. Using a bacterial count of ≥20 minimizes cultures while ensuring accurate detection of urinary tract infections.
Area of Science:
- Clinical Microbiology
- Diagnostic Technology
- Urology
Background:
- Urine culture is standard for diagnosing urinary tract infections but is time-consuming.
- Flow cytometry offers a rapid alternative for analyzing urine constituents.
- Optimizing screening criteria is crucial for efficient laboratory workflows.
Purpose of the Study:
- To evaluate the UF-1000i™ flow cytometer for counting urine bacteria.
- To determine optimal white blood cell (WBC) and bacteria screening criteria to reduce unnecessary urine cultures.
- To ensure high sensitivity for detecting true positive infections.
Main Methods:
- Compared UF-1000i™ flow cytometry results with urine cultures on CHROMagar™ Orientation (CO) medium.
- Analyzed 2496 urine specimens.
- Assessed various combinations of WBC and bacterial counts as screening criteria.
Main Results:
- A bacterial count threshold of ≥20 on UF-1000i™ achieved 92.6% overall sensitivity.
- This criterion allowed 35% of specimens to be excluded from culture.
- Sensitivity for Gram-negative and Gram-positive organisms was 99.2% and 85.0%, respectively.
Conclusions:
- The UF-1000i™ is a reliable method for urine screening.
- Using a bacterial count of ≥20 as the sole screening criterion is effective and efficient.
- This approach simplifies urine analysis and reduces laboratory workload.
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