Related Experiment Video
Updated: Jun 5, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
Smear layer dissolution by peracetic acid of low concentration
G De-Deus1, E M Souza, J R Marins
1Veiga de Almeida University (UVA), Rio de Janeiro, Brazil. endogus@gmail.com
Aim:
To test the effect of a noncaustic concentration of peracetic acid (PAA) in a standardized smear layer model.
Methodology:
The smear layer dissolution kinetics of 0.5% PAA on human dentine were compared to those of 2.25% PAA and 17% ethylenediaminetetraacetic acid (EDTA) solutions. Coronal dentine discs were prepared from six human maxillary molars. A standardized smear layer was produced on the pulpal side of each disc. The smear layer-covered surface was divided into three similar areas and then exposed to one of the three solutions tested. Co-site image sequences (around 40, 500 ×) of the specific areas were obtained after four cumulative demineralisation times (15, 30, 60 and 180 s). An image processing and analysis sequence measured sets of images, providing data of area fraction (AF, dentine-free area in % of total analysis area). A general linear model for repeated measures was used to verify the influence of time and solution type over the change in AF from baseline (ΔAF).
Results:
Overall, EDTA and 2.25% PAA produced higher ΔAF values than the 0.5% PAA solution (P < 0.05). No significant difference was observed in ΔAF between 15 s and 30 s (P > 0.05). After 60 s of etching, all tested solutions produced similar ΔAF (P > 0.05), whereas at 180 s, ΔAF of both EDTA and 2.25% PAA continued to increase (P > 0.05).
Conclusions:
After 60 s of contact, the 0.5% PAA solution dissolved smear layer as well as 2.25% PAA and 17% EDTA.
Related Concept Videos
Theories of Dissolution: Diffusion Layer Model
This process starts with a thin layer, saturated with the drug, forming at the interface between the solid and liquid. The solute then diffuses from this layer into the main solution. The Noyes-Whitney equation suggests that the rate of dissolution relies on the diffusion...
Acid Halides to Carboxylic Acids: Hydrolysis
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic acid...
Leveling Effect and Non-Aqueous Acid-Base Solutions
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):
Acid Halides to Esters: Alcoholysis
