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Updated: Aug 15, 2026

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
07:46

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Published on: June 5, 2012

[Drug resistance and floral distribution of Pseudomonas aeruginosa]

C Y Jia1

  • 1Plastic Surgery Center, Xijing Hospital, Fourth Military Medical University, Xi-an.

Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi = Zhonghua Zheng Xing Shao Shang Waikf [I.E. Waike] Zazhi = Chinese Journal of Plastic Surgery and Burns
|September 1, 1990
PubMed

Insights

Pseudomonas aeruginosa strains showed serious antibiotic resistance. Ceftazidime, norfloxacin, and amikacin were most effective, indicating a need for strengthened isolation measures in burn units.

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Burn wound infections

Background:

  • Pseudomonas aeruginosa is a common opportunistic pathogen in burn wound infections.
  • Antibiotic resistance in P. aeruginosa poses a significant challenge in clinical settings.

Purpose of the Study:

  • To investigate the antibiotic susceptibility patterns of Pseudomonas aeruginosa strains isolated from a burn ward.
  • To identify prevalent serotypes and assess the extent of cross-infection.

Main Methods:

  • Isolation and identification of 100 P. aeruginosa strains from burn patients between June 1986 and September 1989.
  • Antibiotic susceptibility testing and minimum inhibitory concentration (MIC) determination.
  • Serotyping investigation to determine the predominant serotype.

Main Results:

  • High levels of resistance to commonly used antibiotics were observed.
  • Ceftazidime, norfloxacin, and amikacin demonstrated the lowest MIC values, indicating higher susceptibility.
  • Serotype P 11 was identified as the predominant strain, with evidence of cross-infection.

Conclusions:

  • The study highlights a serious antibiotic resistance problem with P. aeruginosa in the burn ward.
  • Ceftazidime, norfloxacin, and amikacin are potential therapeutic options.
  • Strengthened isolation protocols are crucial to control the spread of P. aeruginosa, particularly serotype P 11, and prevent cross-infection.

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