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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 5, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Two-parameter angular light scatter collection for microfluidic flow cytometry by unique waveguide structures.

Jessica Godin1, Yu-Hwa Lo

  • 1Department of Electrical and Computer Engineering, University of California, San Diego, 9500 Gilman Drive MC 0409, La Jolla, CA 92037, USA.

Biomedical Optics Express
|January 25, 2011
PubMed
Summary
This summary is machine-generated.

A novel microfluidic cytometer integrates on-chip illumination and light scatter collection. This simplified optical system achieves good forward scatter for polystyrene beads at high flow rates.

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Last Updated: Jun 5, 2026

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Area of Science:

  • Biomedical Engineering
  • Optical Engineering
  • Analytical Chemistry

Background:

  • Microfluidic cytometers offer miniaturized cell analysis.
  • Integrating optical systems on-chip presents design challenges.
  • Accurate light scatter detection is crucial for cell characterization.

Purpose of the Study:

  • To develop an on-chip microfluidic cytometer with integrated illumination and two-parameter light scatter collection.
  • To simplify the optical system of a microfluidic cytometer.
  • To evaluate the performance of the developed cytometer using polystyrene beads.

Main Methods:

  • Utilized waveguide numerical aperture restrictions and light-blocking elements for optical system design.
  • Integrated illumination and two-parameter light scatter collection systems on a single chip.
  • Employed a simplified optical pathway for enhanced efficiency.
  • Conducted experiments using polystyrene beads at a flow rate of 28 cm/s.

Main Results:

  • Achieved good forward scatter coefficients of variation (9.7-18.3%) for polystyrene beads.
  • Demonstrated successful integration of illumination and light scatter detection on-chip.
  • Validated the performance of the simplified optical system under high flow conditions.

Conclusions:

  • The developed microfluidic cytometer with an integrated, simplified optical system is effective for cell analysis.
  • The design overcomes challenges in on-chip optical integration for microfluidic devices.
  • The system shows promise for high-throughput, miniaturized flow cytometry applications.