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Updated: Jun 5, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Synthesis, characterization, and DNA binding and cleavage properties of
Pulimamidi R Reddy1, Nomula Raju, Battu Satyanarayana
1Department of Chemistry, Osmania University, Hyderabad, India. rabi_pr@rediffmail.com
Abstract:
The mononuclear dipeptide-based Cu(II) complexes [Cu(II) (trp-phe)(phen)(H₂O)] ⋅ ClO₄ (1) and [Cu(II) (trp-phe)(bpy)(H₂O)] ⋅ ClO₄ (2) (trp-phe=tryptophanphenylalanine, phen=1,10-phenanthroline, bpy=2,2'-bipyridine) were isolated, and their interaction with DNA was studied. They exhibit intercalative mode of interaction with DNA. The intercalative interaction was quantified by Stern-Volmer quenching constant (K(sq) =0.14 for 1 and 0.08 for 2). The Cu(II) complexes convert supercoiled plasmid DNA into its nicked circular form hydrolytically at physiological conditions at a concentration as low as 5 μM (for 1) and 10 μM (for 2). The DNA hydrolysis rates at a complex concentration of 50 μM were determined as 1.74 h(-1) (R=0.985) for 1 and 0.65 h(-1) (R=0.965) for 2. The rate enhancement in the range of 2.40-4.10×10⁷-fold compared to non-catalyzed double-stranded DNA is significant. This was attributed to the presence of a H(2) O molecule in the axial position of the Cu complexes.
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