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Published on: July 30, 2018
[Construction of adenoviral vector carrying Smad3D or Smad7]
Jianjun Qin1, Qinghua Zhou, Yang Qin
1Key Laboratory of Sichuan Province, Laboratory of Lung Cancer Molecular Biology, Department of Thoracocardiac Vascular Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Background:
To construct recombinant adenoviral vector carrying Smad3D or Smad7 by a simplified means.
Methods:
Based on AdEasy System, adenoviral backbone plasmid vector and shuttle vector carrying the gene of interest were transferred into E.coli BJ5183 by chemical transformation methods in special order. The homologous recombination was performed.
Results:
Recombinant adenoviral vector pAd-Smad3D and pAd-Smad7 were constructed successfully, which were confirmed by restriction enzyme digesting.
Conclusions:
Recombinant adenoviral vector may be constructed quickly and efficiently in E.coli by sequential chemical transformation methods.
