Related Experiment Video
Updated: Jun 4, 2026

Exploring Protein-Glycan Interactions: Advances in Nuclear Magnetic Resonance
Published on: August 26, 2025
Structural analysis of glycans by NMR chemical shift prediction
Abstract:
Structural determination of N- and O-linked glycans as well as polysaccharides is hampered by the limited spectral dispersion. The computerized approach CASPER, an acronym for computer assisted spectrum evaluation of regular polysaccharides, uses liquid state NMR data to elucidate carbohydrate structure based on agreement with predicted (1)H and (13)C chemical shifts. We here demonstrate developments based on multiple through-bond J-based correlations that significantly enhance the credence to the sequence connectivities proposed in the analysis exemplified by an oligosaccharide and a bacterial polysaccharide. The approach is also suitable for predicting (1)H and (13)C NMR chemical shifts of synthesized oligosaccharides and glycoconjugates, thereby corroborating a proposed structure.
Related Concept Videos
NMR Spectroscopy: Chemical Shift Overview
For instance, the proton...
NMR Spectroscopy Of Amines
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei in a...
¹H NMR Chemical Shift Equivalence: Homotopic and Heterotopic Protons
Carbon-13 (¹³C) NMR: Overview
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

