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Related Experiment Video

Updated: Jun 4, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Rationally designed fluorescence 'turn-on' sensor for Cu2+.

Kyoung Chul Ko1, Jia-Sheng Wu, Hyun Jung Kim

  • 1Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Republic of Korea.

Chemical Communications (Cambridge, England)
|February 2, 2011
PubMed
Summary
This summary is machine-generated.

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A novel coumarin-based sensor, imino-coumarin (IC), selectively detects copper ions (Cu2+) with a strong fluorescence signal. This sensor was successfully used for in vitro and in vivo imaging of copper in cells and organs.

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Copper ions (Cu2+) play crucial roles in biological processes but their dysregulation is linked to various diseases.
  • Developing selective and sensitive detection methods for Cu2+ is essential for biological and environmental monitoring.
  • Fluorescent sensors offer advantages like high sensitivity, rapid response, and real-time monitoring capabilities.

Purpose of the Study:

  • To design and synthesize a novel coumarin-based fluorescent sensor, imino-coumarin (IC).
  • To investigate the selectivity and sensitivity of IC for Cu2+ detection in aqueous solutions.
  • To evaluate the applicability of IC for in vitro and in vivo imaging of Cu2+.

Main Methods:

  • Rational design and synthesis of the imino-coumarin (IC) fluorescent sensor.

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Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
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Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

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Last Updated: Jun 4, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
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Published on: March 13, 2013

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
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Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Published on: October 15, 2019

  • Spectroscopic analysis (fluorescence spectroscopy) to determine sensor performance.
  • Density Functional Theory (DFT) and Time-Dependent DFT (TDDFT) calculations to elucidate the sensing mechanism.
  • Microscopic imaging (in vitro and in vivo) for cellular and organ-level Cu2+ detection.

    • Main Results:

    • The synthesized IC sensor exhibited high selectivity for Cu2+ over other metal ions.
    • A significant fluorescence 'turn-on' response was observed upon binding with Cu2+.
    • DFT/TDDFT calculations indicated that fluorescence enhancement is due to the blocking of electron transfer from the nitrogen lone pair upon Cu2+ complexation.
    • IC was successfully applied for microscopic imaging of Cu2+ in LLC-MK2 cells and living organs.

    Conclusions:

    • Imino-coumarin (IC) is a highly selective and sensitive fluorescent sensor for Cu2+ detection.
    • The mechanism involves the inhibition of photoinduced electron transfer upon Cu2+ coordination.
    • IC demonstrates significant potential for biological imaging applications, enabling visualization of Cu2+ in cellular and in vivo models.