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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Updated: Jun 4, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

ChIP-on-chip for FoxP3.

Ye Zheng¹

¹Nomis Center for Immunobiology and Microbial Pathogenesis, The Salk Institute for Biological Studies, La Jolla, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 3, 2011

Summary

This summary is machine-generated.

Regulatory T (Treg) cells maintain immune balance. Researchers identified key genes directly regulated by the Foxp3 transcription factor, revealing new insights into Treg cell function and autoimmune disease development.

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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction

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Published on: January 22, 2020

Area of Science:

Immunology
Molecular Biology
Genetics

Background:

Regulatory T (Treg) cells are crucial for immune suppression and homeostasis.
The transcription factor Foxp3 is essential for Treg cell development and function.
Foxp3 mutations lead to severe immunodeficiency and autoimmune diseases.

Purpose of the Study:

To identify direct downstream targets of Foxp3 in Treg cells.
To understand the regulatory network controlled by Foxp3.

Main Methods:

Chromatin immunoprecipitation (ChIP).
Mouse whole genome tiling array profiling (ChIP-on-Chip).

Main Results:

Foxp3 directly controls the expression of several Treg signature molecules.
Foxp3 regulates transcription factors that govern other Treg-specific genes.
Identified a network of genes critical for Treg cell function.

Conclusions:

Foxp3 acts as a master regulator of Treg cell identity and function.
Understanding Foxp3 targets provides insights into autoimmune disease mechanisms.
This study elucidates key molecular pathways in immune regulation.