Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular basis of polyadenylated RNA fate determination in the nucleus.

Nature·2026
Same author

Mechanism of RACK1-dependent ZAKα activation at stalled and collided ribosomes.

Molecular cell·2026
Same author

TENT-5 polyadenylates and regulates male-specific transcripts in Caenorhabditis elegans.

G3 (Bethesda, Md.)·2026
Same author

Degron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism.

Communications biology·2026
Same author

DIS3 mutations enhance AID-driven translocations during B-cell activation, promoting transformation to multiple myeloma.

Nature communications·2026
Same author

PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA.

Nucleic acids research·2026

Related Experiment Video

Updated: Jun 4, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

The eukaryotic RNA exosome: same scaffold but variable catalytic subunits.

Søren Lykke-Andersen1, Rafal Tomecki, Torben Heick Jensen

  • 1Department of Molecular Biology, Centre for mRNP Biogenesis and Metabolism, Aarhus University, Aarhus C, Denmark.

RNA Biology
|February 4, 2011
PubMed
Summary

The RNA exosome, a protein complex for RNA processing, has varying catalytic subunits. Recent findings reveal differential composition and location of exosome variants across eukaryotes.

More Related Videos

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The RNA exosome is a crucial ribonucleolytic complex involved in RNA processing and degradation.
  • It comprises a core of nine subunits and associated catalytic subunits/co-factors that determine its function.
  • The *Saccharomyces cerevisiae* exosome is a well-studied model for eukaryotic exosomes.

Purpose of the Study:

  • To review recent findings on the catalytic subunits of eukaryotic RNA exosomes.
  • To discuss variations in exosome composition and subcellular localization.
  • To highlight the differential catalytic potential between nuclear and cytoplasmic exosome versions.

Main Methods:

  • Literature review of recent discoveries.
  • Comparative analysis of exosome structures and functions across eukaryotes.
  • Discussion of experimental findings from various laboratories.

Main Results:

  • Eukaryotic exosomes exhibit variations in their catalytic subunits and overall composition.
  • Subcellular localization (nuclear vs. cytoplasmic) influences the exosome's catalytic activity.
  • The catalytic potential is not solely dependent on the core complex but also on associated factors.

Conclusions:

  • Eukaryotic RNA exosomes are diverse, with variations in catalytic subunits and subcellular distribution.
  • Differential composition allows for specialized roles in RNA metabolism.
  • Understanding these variations is key to comprehending RNA processing and degradation pathways.