Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heavy chain variable region gene utilization in human antibodies.

V Pascual1, J Andris, J D Capra

  • 1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048.

International Reviews of Immunology
|January 1, 1990
PubMed
Summary

Human antibody sequencing reveals a skewed distribution of heavy chain variable (VH) gene families and segments. This suggests specific VH gene usage patterns in antibody development, with potential implications for immune response understanding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SEA 2022 Standards for Global Control of Cardiovascular Risk.

Clinica e investigacion en arteriosclerosis : publicacion oficial de la Sociedad Espanola de Arteriosclerosis·2022
Same author

Biomedical image classification made easier thanks to transfer and semi-supervised learning.

Computer methods and programs in biomedicine·2020
Same author

DeepClas4Bio: Connecting bioimaging tools with deep learning frameworks for image classification.

Computers in biology and medicine·2019
Same author

Antibiogramj: A tool for analysing images from disk diffusion tests.

Computer methods and programs in biomedicine·2017
Same author

Bloodstream infections caused by Escherichia coli producing AmpC β-lactamases: epidemiology and clinical features.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2016
Same author

Different Gene Expression Signatures in Children and Adults with Celiac Disease.

PloS one·2016

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Human antibodies are crucial for adaptive immunity.
  • The variable regions of antibodies (VH) determine antigen specificity.
  • Understanding VH gene usage is key to antibody repertoire analysis.

Purpose of the Study:

  • To sequence the heavy chain variable regions of human antibodies.
  • To analyze the distribution of VH gene families and segments.
  • To investigate potential biases in VH gene usage.

Main Methods:

  • Polymerase chain reaction (PCR) sequencing of heavy chain variable regions.
  • Analysis of over thirty human antibodies with defined specificities.
  • Statistical analysis of VH gene family and segment distribution.

Related Experiment Videos

Main Results:

  • Sequencing of over thirty human antibody VH regions was performed.
  • A disproportionate skew towards recently discovered VH gene families was observed.
  • Non-uniform usage of individual gene segments within VH families was identified.

Conclusions:

  • The human antibody repertoire exhibits biased usage of VH gene families and segments.
  • These findings suggest regulatory mechanisms influencing VH gene selection.
  • Further research is needed to elucidate the functional and evolutionary implications of these biases.