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CRISPR-Cas-mediated Multianalyte Synthetic Urine Biomarker Test for Portable Diagnostics
Published on: December 8, 2023
[Evolution of strategy to measure urinary supersaturation]
Nobuyo Morita1, Manabu Moriyama, Katsuhito Miyazawa
1The Department of Urology, Kanazawa Medical University.
Hinyokika Kiyo. Acta Urologica Japonica
|February 10, 2011
Summary
This study validates a microplate method for measuring the metastable limit (ML) of urinary stones. This simple test correlates with urinary calcium levels, aiding in preventing stone recurrence.
Area of Science:
- Nephrology
- Urology
- Biochemistry
Context:
- Urinary stones affect a significant portion of the population, with higher lifetime morbidity in males.
- Current methods for assessing stone risk can be complex.
- A simple, reliable method to assess urinary stone risk is needed.
Purpose:
- To evaluate the usability of a microplate-based method for measuring the metastable limit (ML) of urinary stones.
- To determine the correlation of ML with urinary stone risk factors.
Summary:
- The microplate method for measuring metastable limit (ML) was confirmed as usable.
- ML showed a correlation with urinary calcium concentration in healthy subjects, single stone formers, and recurrent stone formers.
- ML correlated with urinary oxalate and citrate in healthy subjects, but not recurrent stone formers.
Impact:
- The metastable limit (ML) measurement is a simple and easy method to assess urinary calcium levels.
- This microplate assay could serve as a valuable tool in outpatient settings.
- It offers a convenient indicator for the prevention of urinary stone recurrence.
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