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[Study on high-rate and low-rate metastatic large-cell lung cancer cell lines via differential display reverse
Qing Tian1, Liang'an Chen, Youning Liu
1Department of Pulmonary Medicine, General Hospital of PLA, Beijing 100853, P.R.China.
Background:
To investigate the differentially expressed gene profile between high-rate and low-rate metastatic large-cell lung cancer cell lines via differential display reverse transcript PCR (DDRT-PCR).
Methods:
DDRT-PCR was performed in two large-cell lung cancer cell lines with different metastatic ability. The differentially displayed cDNA were cloned and sequenced and compared with the sequences in the GenBank.
Results:
Fifty differentially displayed cDNAs were acquired, including 18 function-known genes, 16 function-unknown genes, 16 genomic DNA fragments and 4 ESTs.
Conclusions:
The cell lines have different gene expression profiles, and metastasis is affected by multi-gene expression and regulation. Some genes which contribute to the metastasis of lung cancer could be found by DDRT-PCR, and this study gives some new clues to cancer diagnosis and therapy.
