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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Design of a normal incidence multilayer imaging x-ray microscope.

D L Shealy1, D R Gabardi, R B Hoover

  • 1Department of Physics, University of Alabama at Birmingham, Birmingham, Alabama 35294.

Journal of X-Ray Science and Technology
|February 11, 2011
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Doubly reflecting multilayer x-ray optics are feasible, enabling high-resolution solar imaging. This technology paves the way for advanced aplanatic imaging soft x-ray microscopes for diverse scientific applications.

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Area of Science:

  • X-ray optics and instrumentation
  • Solar physics
  • Microscopy

Background:

  • Normal incidence multilayer Cassegrain x-ray telescopes were successfully deployed.
  • These instruments provided high spatial resolution solar images.
  • Feasibility of doubly reflecting multilayer x-ray optical systems was demonstrated.

Purpose of the Study:

  • To design a doubly reflecting normal incidence multilayer imaging x-ray microscope.
  • To achieve aplanatic imaging using multilayer optics technology.
  • To explore applications in advanced scientific research.

Main Methods:

  • Design based on the Schwarzschild configuration using two spherical mirrors.
  • Optimization of mirror curvature to minimize spherical aberration and coma.
  • Optical system ray trace analysis to predict performance.

Main Results:

  • Ray trace analysis indicates diffraction-limited performance.
  • Predicted spatial resolution of 600 Å over a 1 mm field of view at 100 Å wavelength.
  • Microscope designs are currently in fabrication.

Conclusions:

  • Aplanatic imaging soft x-ray microscopes are achievable with multilayer optics.
  • These microscopes offer significant potential for x-ray astronomy, lithography, and research.
  • Further applications include biological, biomedical, metallurgical, and laser fusion research.