[TCRζ gene expression in TCR gene-modified T cells specific for DLBCL-associated antigen]

To analyze the expression level of TCRζ chain gene in the DLBCL-associated antigen-specific T cells before and after being activated by coculture with Toledo cells (DLBCL cell line).

Real-time PCR with SYBR GreenI technique was used for detecting TCRζ chain expression in activated and unactivated DLBCL-associated antigen-specific T cells. β2 microglobulin gene (β2M) was used as an endogenous reference. Relative mRNA expression level of TCRζ gene was analyzed by the formula of both 2(-δCt); ×100% and 2(-δδCt);.

Compared with (1.74±0.28)% of the relative mRNA expression level of TCRζ gene in TCR gene-untransduced T cells, the expression level of TCRζ gene was (1.78±0.22)% in unactivated TCR gene-transduced T cells and showed no obvious increase. While the expression of TCRζ gene arrived at (11.54±1.98)% in the activated TCR gene-modified T cells, which was significantly higher than that in unactivated TCR gene-modified and TCR gene-untransduced T cells (P<0.05), and was increased (6.59±0.80) and (6.48±0.36) times, respectively.

The expression of TCRζ chain was up-regulated, when TCR gene-modified T cells were activated by the stimulation of specific antigens.