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Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Viral Hepatitis I: Introduction01:28

Viral Hepatitis I: Introduction

Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...

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Related Experiment Video

Updated: Jun 4, 2026

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

Hepatitis E virus cell culture models.

Hiroaki Okamoto1

  • 1Division of Virology, Department of Infection and Immunity, Jichi Medical University, School of Medicine, Tochigi-Ken, Japan. hokamoto@jichi.ac.jp

Virus Research
|February 15, 2011
PubMed
Summary
This summary is machine-generated.

Efficient cell culture systems for hepatitis E virus (HEV) have been developed, enabling propagation of various HEV strains. The ORF3 protein is crucial for HEV virion egress from infected cells.

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Area of Science:

  • Virology
  • Hepatology
  • Cell Biology

Background:

  • Hepatitis E virus (HEV) propagation in cell culture has historically been inefficient.
  • Previous attempts faced challenges in achieving robust replication of diverse HEV strains.

Purpose of the Study:

  • To review the development of efficient cell culture systems for various HEV strains.
  • To elucidate the role of HEV ORF3 protein in virion egress using cell culture models.

Main Methods:

  • Establishment of efficient HEV cell culture systems using PLC/PRF/5 and A549 cells with high-titer fecal suspensions (genotypes 3 and 4).
  • Construction and utilization of a full-length infectious cDNA clone (pJE03-1760F/wt) for HEV replication studies.
  • Generation and analysis of an ORF3-deficient HEV mutant to investigate virion egress.

Main Results:

  • Efficient propagation of multiple HEV strains, including clinical isolates and strains from blood, in PLC/PRF/5 and A549 cells through serial passages.
  • Demonstration that the HEV ORF3 protein is essential for virion egress and is associated with lipid-enveloped particles on the cell surface.
  • Successful replication of HEV using both infectious fecal material and a full-length infectious cDNA clone.

Conclusions:

  • Development of robust cell culture systems facilitates the study of a wide range of HEV strains.
  • The HEV ORF3 protein plays a critical role in the release of infectious virions from host cells.
  • Cell culture models provide valuable insights into HEV replication and pathogenesis, particularly virion egress.