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Related Experiment Video

Updated: Jun 4, 2026

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
07:45

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Chemical screening with zebrafish embryos.

Hanbing Zhong1, Shuo Lin

  • 1Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen University Town, Shenzhen, China. zhong@szpku.edu.cn

Methods in Molecular Biology (Clifton, N.J.)
|February 15, 2011
PubMed
Summary

Zebrafish are excellent for discovering new functional chemicals due to their transparency and rapid development. Researchers used this model to identify and characterize two novel Smo inhibitors, advancing molecular biology tools.

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Chemical Biology

Background:

  • Functional chemicals are vital for molecular biology research.
  • Zebrafish offer unique advantages as an in vivo model for chemical screening, including transparency and rapid embryonic development.
  • Zebrafish-based chemical screens, established in 2000, have successfully identified numerous bioactive compounds.

Purpose of the Study:

  • To detail the methodology of a typical zebrafish-based chemical screen.
  • To exemplify the protocol using the identification and characterization of novel Smo inhibitors.
  • To highlight the utility of zebrafish for evaluating developmental phenotypes and discovering functional chemicals.

Main Methods:

  • Utilizing zebrafish embryos for in vivo chemical compound screening.

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Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

Development of automated imaging and analysis for zebrafish chemical screens.
10:49

Development of automated imaging and analysis for zebrafish chemical screens.

Published on: June 24, 2010

Related Experiment Videos

Last Updated: Jun 4, 2026

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
07:45

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

Development of automated imaging and analysis for zebrafish chemical screens.
10:49

Development of automated imaging and analysis for zebrafish chemical screens.

Published on: June 24, 2010

  • Employing a Gli:GFP transgenic zebrafish line for identifying Smo pathway modulators.
  • Microscopic examination of developmental phenotypes to assess chemical compound effects.
  • Characterization of identified functional chemicals, specifically Smo inhibitors.

    • Main Results:

    • Successful identification of two novel Smo inhibitors through a zebrafish chemical screen.
    • Demonstration of the effectiveness of zebrafish embryogenesis for evaluating compound activity.
    • Characterization of the identified Smo inhibitors provides new tools for molecular biology.

    Conclusions:

    • Zebrafish provide a powerful and comprehensive platform for in vivo chemical screens.
    • The described protocol enables the discovery and characterization of novel functional chemicals.
    • This approach enhances the identification of targeted molecular modulators, such as Smo inhibitors.