Nuclear envelope dynamics during plant cell division suggest common mechanisms between kingdoms
Katja Graumann1, David E Evans
1School of Life Sciences, Oxford Brookes University, Oxford OX3 0BP, UK.
The Biochemical Journal
|February 18, 2011
Summary
Plant nuclear envelope (NE) behavior during mitosis was studied using novel markers. Findings suggest the ER-retention theory applies to plants and reveal unique NE reformation patterns.
Area of Science:
- Plant cell biology
- Molecular and cell biology
- Genetics and genomics
Background:
- Nuclear envelope (NE) behavior during open mitosis is well-studied in metazoans.
- Investigating plant NE dynamics is challenging due to a lack of native markers.
Purpose of the Study:
- To investigate the behavior of the nuclear envelope (NE) during plant mitosis.
- To determine if the ER-retention theory applies to plant NE membranes.
- To identify unique features of plant NE dynamics during cell division.
Main Methods:
- Utilized living synchronized tobacco BY-2 suspension cultures.
- Employed non-functional lamin B receptor-green fluorescent protein (LBR-GFP) and functional native proteins, Arabidopsis thaliana SUN1 (AtSUN1) and AtSUN2.
- Tracked protein mobility during various cell division stages.
Main Results:
- Provided evidence that the endoplasmic reticulum (ER)-retention theory is applicable to plant NE membranes.
- Observed NE membrane components closely associated with chromatin throughout division.
- Documented spatially distinct NE reformation, starting at chromatin surfaces near spindle poles and ending at the cell plate-facing surface.
- Suggested a role for AtSUN2 in NE breakdown.
Conclusions:
- The ER-retention theory is applicable to plant nuclear envelope membranes.
- Unique plant-specific features of NE dynamics during mitosis were identified.
- AtSUN2 may play a role in nuclear envelope breakdown during plant cell division.
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