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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 4, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Remote focusing for programmable multi-layer differential multiphoton microscopy.

Erich E Hoover, Michael D Young, Eric V Chandler

    Biomedical Optics Express
    |February 18, 2011
    PubMed
    Summary
    This summary is machine-generated.

    We developed remote focusing for multiphoton microscopy, enabling simultaneous multi-layer imaging. This technique allows programmable focal plane control for advanced 3D imaging in scattering samples.

    Keywords:
    (030.5260) Photon counting(170.0180) Microscopy(180.4315) Nonlinear microscopy(180.5810) Scanning microscopy(180.6900) Three-dimensional microscopy

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    Last Updated: Jun 4, 2026

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
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    07:42

    Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

    Published on: February 24, 2026

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Microscopy

    Background:

    • Multiphoton laser scanning microscopy (MLSM) is a powerful technique for deep tissue imaging.
    • Achieving simultaneous multi-layer imaging in MLSM remains challenging, limiting volumetric imaging capabilities.

    Purpose of the Study:

    • To introduce and demonstrate remote focusing for simultaneous, programmable multi-layer imaging in MLSM.
    • To enable efficient volumetric imaging in scattering specimens.

    Main Methods:

    • Application of remote focusing to control axial location of multiple focal planes.
    • Simultaneous imaging of multiple focal planes using single element detection.
    • Synchronization of remote focusing with lateral scanning for orthogonal plane imaging.

    Main Results:

    • Demonstrated simultaneous, programmable multi-layer imaging capability.
    • Facilitated volumetric imaging in scattering specimens.
    • Showcased scalability to a large number of focal planes.
    • Enabled imaging in orthogonal scan planes by synchronizing with lateral scans.

    Conclusions:

    • Remote focusing is a versatile technology for advancing MLSM.
    • This method significantly enhances volumetric imaging capabilities in scattering media.
    • The technique offers practical scalability for complex imaging applications.