Related Experiment Video
Updated: Jun 4, 2026

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
Published on: May 1, 2019
[Expression of cancer-testis antigens in human lung cancer]
Hui Zhao1, Chenyang Shen, Danlei Wang
1Department of Thoracic Surgery, People's Hospital, Peking University, Beijing 100044, P.R.China.
Background:
To investigate the expression of cancer-testis antigens(CTA) in human lung cancer.
Methods:
Reverse-transcription polymerase chain reaction (RT-PCR) was used to investigate the expression of the MAGE-1, -3, SSX-1, -2, -4, -5 and NY-ESO-1 genes in 35 lung cancer samples and corresponding non-tumorous lung tissues. Three samples selected randomly from each CTA PCR product were sequenced.
Results:
In 35 tumor samples, the MAGE-1, -3, SSX-1, -2, -4, -5 and NY-ESO-1 mRNA expression rates were 34.3%(12/35), 57.1%(20/35), 17.1%(6/35), 17.1%(6/35), 20.0%(7/35), 25.7%(9/35) and 37.1%(13/35), respectively. The positive rate was 74.3%(26/35) for at least one of these genes expression, and 65.7%(23/35) for two or more genes coexpression. No non-tumorous lung tissue was positive for these genes. The DNA sequence confirmed that the RT-PCR products were truly CTA cDNA.
Conclusions:
The cancer-testis antigens are potential targets for antigen-special immunotherapy of lung cancer. The coexpression pattern of these antigens provides a theoretic foundation for developing a polyvalent lung cancer vaccine.
Related Concept Videos
lncRNA - Long Non-coding RNAs
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

