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Isolation of DNA and RNA

D J Perry1

  • 1Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free Hospital School of Medicine, London, UK.

Methods in Molecular Medicine
|February 23, 2011
PubMed

Blood samples for most coagulation tests are collected into 3.8% trisodium citrate in a ratio of 1 part anticoagulant to 9 parts blood. Whole-blood samples for DNA isolation can be stored at -50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats-the interface between the red cells and the plasma that is seen following centrifugation of whole blood. This latter method also allows isolation of the plasma fraction.

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