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Updated: Jun 4, 2026
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A Rabbit Model of Durable Transgene Expression in Jugular Vein to Common Carotid Artery Interposition Grafts
Published on: September 10, 2018
Gene delivery to rabbit arteries using the collar model.
M O Hiltunen1, M P Turunen, S Ylä-Herttuala
1AI Virtanen Institute, University of Kuopio, Kuopoi, Finland.
Methods in Molecular Medicine
|February 23, 2011
Summary
Vascular gene therapy utilizes molecular pathology insights for new treatments. Both extravascular and intravascular delivery methods can achieve sufficient gene expression in vessel walls for therapeutic effects.
Area of Science:
- Vascular biology and gene therapy
- Molecular mechanisms of vascular disease
Background:
- Emerging molecular pathology of vascular diseases offers novel targets for gene therapy.
- Gene delivery to the vessel wall is crucial for effective vascular gene therapy.
- Both extravascular and intravascular approaches can achieve therapeutic gene expression.
Purpose of the Study:
- To describe two gene delivery methods for vascular gene transfer experiments.
- To illustrate controlled gene transfer using the collar model.
Main Methods:
- Extravascular gene delivery using plasmid DNA.
- Intravascular gene delivery using plasmids and adenoviruses.
- Utilizing a collar model for controlled gene transfer experiments.
Main Results:
- Extravascular plasmid DNA delivery achieves high transfection efficiency.
- Both plasmid DNA and adenoviral vectors are suitable for extravascular and intravascular delivery.
- The collar model enables precise control over gene transfer in vascular studies.
Conclusions:
- Effective gene expression in the vessel wall is achievable through both extravascular and intravascular gene delivery.
- Plasmid DNA and adenoviruses are versatile tools for vascular gene therapy.
- Controlled gene transfer methods are essential for studying vascular gene therapy efficacy.
