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Related Experiment Video

Updated: Jun 4, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
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Measuring Ca²+ changes in multiwell format using the Fluorometric Imaging Plate Reader.

Ian C B Marshall1, Davina E Owen, Shaun McNulty

  • 1Neurology Centre of Excellence for Drug Discovery, GlaxoSmithKline Pharmaceuticals Ltd., Harlow, Essex, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

The Fluorometric Imaging Plate Reader (FLIPR) revolutionizes drug discovery by enabling simultaneous, high-speed measurement of cellular responses. This high-throughput screening tool efficiently analyzes compound libraries for drug development.

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Area of Science:

  • Pharmacology
  • Biotechnology
  • Assay Development

Background:

  • The Fluorometric Imaging Plate Reader (FLIPR) has been instrumental in pharmaceutical drug discovery for nine years.
  • Conventional plate readers lack the simultaneous multi-well measurement and high temporal resolution of FLIPR.

Purpose of the Study:

  • To highlight the significant contributions of FLIPR technology to drug discovery programs.
  • To detail the advantages of FLIPR for screening compound libraries and identifying drug candidates.

Main Methods:

  • Utilizing FLIPR for simultaneous fluorescence emission measurement from 96- or 384-well plates.
  • Recording dynamic intracellular processes, including Ca(2+) ion concentration, membrane potential, and pH changes.
  • Employing FLIPR to assess functional cellular responses to compounds.

Main Results:

  • FLIPR enables rapid distinction between full agonists, partial agonists, and antagonists.
  • The system facilitates high-throughput screening, capable of analyzing over 50,000 compounds daily in a 384-well format.
  • FLIPR provides high temporal resolution for dynamic cellular process monitoring.

Conclusions:

  • FLIPR is a valuable tool for drug discovery, significantly enhancing the efficiency of compound library screening.
  • Its ability to measure dynamic cellular responses in real-time makes it ideal for identifying novel therapeutic agents.