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Related Experiment Video

Updated: Jun 4, 2026

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Localized, targeted, and sustained siRNA delivery.

Melissa D Krebs¹, Eben Alsberg

¹Case Western Reserve University, Biomedical Engineering, 309 Wickenden, 10900 Euclid Avenue, Cleveland, OH, USA.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|February 23, 2011

Summary

Localized delivery of short interfering RNA (siRNA) is crucial for treating various diseases. Researchers are exploring diverse methods, including nanoparticles and biomaterial scaffolds, to enhance siRNA

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High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

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Last Updated: Jun 4, 2026

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

Published on: September 13, 2012

Area of Science:

Molecular Biology
Biotechnology
Drug Delivery Systems

Background:

Short interfering RNA (siRNA) is a molecule that silences gene expression within the cytoplasm by forming an RNA-induced silencing complex (RISC).
Effective localized delivery of siRNA in vivo remains a significant challenge across numerous disease states, including cancers, HIV, neurodegenerative disorders, and cardiovascular diseases.

Purpose of the Study:

To explore and evaluate various modalities for the localized delivery of siRNA to specific target sites.
To review current strategies and emerging approaches for overcoming challenges in siRNA delivery for therapeutic applications.

Main Methods:

Direct injection of naked siRNA into specific tissues like the eye, central nervous system, and lung.
Chemical modification of siRNA to improve stability and cellular uptake.
Complexation of siRNA with liposomes or polymers to create nanoparticles for enhanced delivery.
Utilizing macroscopic biomaterial scaffolds as a novel approach for localized siRNA delivery.

Main Results:

Naked siRNA injection has demonstrated successful gene silencing in specific tissues (eye, CNS, lung).
Chemical modifications and nanoparticle formulations (liposomes, polymers) show promise in stabilizing siRNA and facilitating uptake in other tissues.
Biomaterial scaffolds represent a recent and developing area for localized siRNA delivery, requiring further optimization.

Conclusions:

Localized siRNA delivery is essential for treating a range of systemic diseases.
A variety of methods, including direct injection, chemical modification, nanoparticles, and biomaterial scaffolds, are being investigated to improve siRNA delivery efficacy.
Further research into optimizing these delivery systems, particularly biomaterial scaffolds, holds significant potential for advancing RNAi-based therapeutics.