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Updated: Jun 4, 2026
10:52
Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
Published on: May 25, 2019
In Vitro Fertilization of Astyanax mexicanus
1Department of Biology, New York University, NY 10003, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONThis protocol describes an alternative method for breeding Astyanax mexicanus, using in vitro fertilization. Sperm collected from the male and eggs collected from the female are placed in a Petri dish, and sperm are activated by the addition of fresh system water. The eggs are observed under low magnification for signs of fertilization, usually marked by the onset of cleavage.
Related Concept Videos
01:24In Vitro Fertilization
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
01:38Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...