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Updated: Jun 4, 2026

RNAi Interference by dsRNA Injection into Drosophila Embryos
Published on: April 11, 2011
Preparation of Double-Stranded RNA for Drosophila RNA Interference (RNAi)
Abstract:
INTRODUCTIONRNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. It has been used in our laboratory to phenocopy a series of known mutations in Drosophila, including twist, engrailed, daughterless, Dmef2, and, to a lesser extent, white in the adult eye. This protocol describes the preparation of dsRNA by in vitro transcription of complementary strands of a cloned DNA fragment that codes for all or a portion of the gene of interest, followed by annealing of the transcribed RNA.
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