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Updated: Jun 4, 2026

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
Published on: October 1, 2007
Immunohistochemistry on sections of plant tissues using alkaline-phosphatase-coupled secondary antibody
Abstract:
INTRODUCTIONLike RNA, proteins can be localized in situ on tissue sections. In situ localization or immunohistochemistry provides information on the cellular and subcellular distribution of proteins in different tissues. This protocol describes an alternative to the use of ABC (a preformed Avidin:Biotinylated enzyme Complex used in conjunction with a biotinylated secondary antibody) for non-fluorescent detection in immunohistochemistry. Here, an alkaline-phosphatase-coupled secondary antibody is used with colorimetric substrates for antigen detection.
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