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Updated: Jun 4, 2026
Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
Published on: August 30, 2007
Dissection of rat brains
Abstract:
INTRODUCTIONIn this protocol, the perfused brain of a rat is separated from the surrounding tissue and post-fixed in a formalin/sucrose solution in preparation for freezing and sectioning. Dissection should be done as soon as possible after perfusion to prevent desiccation of the brain. This procedure can also be used to dissect fresh (non-perfused) brains, for example, for Golgi-Cox stain or neurochemical assays.
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