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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Related Experiment Video

Updated: Jun 4, 2026

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
09:38

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Published on: November 23, 2016

Paraffin embedding tissue samples for sectioning.

Andrew H Fischer, Kenneth A Jacobson, Jack Rose

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details paraffin embedding for tissue sectioning, a method yielding superior cell morphology despite requiring extensive processing steps.

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    Last Updated: Jun 4, 2026

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    The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
    05:00

    The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

    Published on: September 5, 2018

    Area of Science:

    • Histology and tissue processing

    Background:

    • Paraffin embedding is a standard technique in histology.
    • Achieving high-quality tissue sections is crucial for accurate microscopic analysis.

    Purpose of the Study:

    • To describe a detailed protocol for embedding tissues in paraffin blocks.
    • To highlight the advantages of paraffin sections for morphological studies.

    Main Methods:

    • Tissue fixation and processing.
    • Embedding tissues in molten paraffin wax.
    • Sectioning of paraffin-embedded tissues.

    Main Results:

    • Paraffin sections offer superior cellular and tissue morphology.
    • The method allows for detailed examination of tissue architecture.

    Conclusions:

    • Paraffin embedding is a valuable technique for obtaining high-resolution tissue sections.
    • This protocol provides a reliable method for histological analysis.